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HomeHomeXTensionsXTensionsRequestsRequests#075 XTENSION REQUEST - how to calculate the volume of an object using voxel number#075 XTENSION REQUEST - how to calculate the volume of an object using voxel number
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#075 XTENSION REQUEST - how to calculate the volume of an object using voxel number
 
I used ziess confocal to get images. The image property x: 0.045, y: 0.045, z:0.649. The voxel number of one object analyzed by imaris is 16. The volume I obtained is 0.021 um3 by 0.045 um x 0.045 um x 0.649 um x 16 However, the volume of this object analyzed by Imaris is 0.0111

 
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Dear Jan,
what the voxel number of a surface object indicates is with how many voxels of the dataset your surface is intersecting. If you would have a perfect rectangle object filling all the 16 voxels, you would have the volume as you calculated. The edges of your surface object is probably filling sub-voxels, especially that your Z step is quite high (undersampled image). The volume is calculated within the surface object which is composed of triangles. Therefore the volume which Imaris computes for your surface object will be accurate.
 
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