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HomeHomeXTensionsXTensionsRequestsRequests#071 XTENSION REQUEST - XTSurfacesSplitIntoSurfaces#071 XTENSION REQUEST - XTSurfacesSplitIntoSurfaces
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#071 XTENSION REQUEST - XTSurfacesSplitIntoSurfaces
 
this extension to be similar to the XTSpotsSplitIntoSurfaces, but it would split surfaces into surface

 
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This could be done from "Image Processing-Surface Functions-Surface Split".
 
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mendel wrote:
This could be done from "Image Processing-Surface Functions-Surface Split".Sat, 06 Jan 2018 09:18:25 GMT

 HI Mendel

the Surface-Split function splits all the surfaces within a surface object  into separate surfaces.   I want a function that is similar to the XTSpotsSplitintoSurfaces function,  but it would split surfaces into a surface instead of spots into a surface. 

I have some larger surface objects that I am using a ROI's. I have another set of surfaces that lie within ROI Surfaces

the function would create a new subset of the surfaces that contain only the surfaces that lie inside the larger ROI surfaces

 
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Hi Jon,

I got your idea. Did you mean grouping the surfaces from a surface object A into subgroups by whether they falls into one large ROI which is one of the several ROI-surfaces that forms another surface object B? 

How about the sub-surface in surface A that falls partially into more than one ROIs of surface B? Do you want to split that sub-
surface further into different ROIs, or delete that sub-surface? If split, then this could be done by several steps:


(1) For  surface object B, run SurfaceSplit to split ROIs into seperate surface objects.

(2)For each split ROI surface object, run surface-surface colocalization with surface object A, then get surface that lies into that ROI

 
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This is also something that we would be interested in. The SplitSpotsintoSurfaces XT is marvelous for interrogating intracellular Spots. If we could use Surfaces instread of Spots it would be a real improvement in what we can do (restricted to circular intracellular items).

The surface-surface coloc idea is what I would do if I had the time, and a cytoplasmic marker of the cells (we used membrane). Here you could select for + + objects from 2 channels to selectively find intracellular Surfaces.

Best of luck and please update on how this goes!

Best wishes,

Darren
 
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I modified the "SplitSpotsintoSurfacesXT" into a new version "SplitSurfacesintoSurfacesXT", which splits the surfaces into surfaces. the function creates a new subset of surfaces that contain only the surfaces that lie inside the larger surfaces
 
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